Radioisotopes Research Today is a free monthly online journal that collates and summarizes the latest research about Radioisotopes, including details on radioimmunotherapy, radionuclides, procedures, hazards.

A radioisotope label-free alpha-bungarotoxin-binding assay using BIAcore sensor chip technology for real-time analysis.

Paulo JA, Hawrot E

Graduate Program in Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, RI 02912, USA.

alpha-Bungarotoxin (alpha-bgtx)-binding proteins, including certain nicotinic acetylcholine receptors and acetylcholine-binding proteins (AChBPs), are frequently characterized with radioisotope-labeled alpha-bgtx-binding assays. Such assays, however, preclude investigations of binding interactions in real time and are hampered by the inconveniences associated with radioisotope-labeled reagents. We used surface plasmon resonance-based technology (BIAcore) to investigate the binding of recombinant AChBP to CM-5 sensor chip surfaces with directly immobilized alpha-bgtx. We validated our BIAcore results by comparing the same biological samples using the traditional (125)I-labeled alpha-bgtx-binding assay. An alpha-bgtx sensor chip, as described here, enables detailed, real-time, radioisotope-free interaction studies that can greatly facilitate the characterization of novel alpha-bgtx-binding proteins and complexes.

Published 29 April 2009 in Anal Biochem, 389(1): 86-8.
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Articles on Radioisotopes published 27 April 2009:

Interventional sialendoscopy for treatment of radioiodine-induced sialadenitis.   Laryngoscope, 119(5): 864-7.

OBJECTIVES/HYPOTHESIS: The purpose of this study is to review our experience with sialendoscopy for the management of radioiodine-induced sialadenitis. METHODS: Retrospective chart review of all patients with radioiodine-induced sialadenitis treated with interventional sialendoscopy. RESULTS: Twelve female patients with a mean age of 46.5 years (range, 25-77 years) underwent interventional sialendoscopy for the treatment of recalcitrant sialadenitis from radioiodine-induced damage to the major ... [Abstract] [Full-text]

Role of magnetite and humic acid in radionuclide migration in the environment.   J Contam Hydrol, 106(3): 144-9.

Sorption of (137)Cs, (90)Sr, (154)Eu and (141)Ce by magnetite has been studied at varying pH (4 to 11) in the presence and absence of humic acid. The sorption studies have also been carried out at varying ionic strength (0.01 to 0.2 M NaClO(4)) and humic acid concentration (2 to 20 mg/L). Percentage sorption of (137)Cs and (90)Sr was found to be pH dependent, with the sorption increasing with increasing pH of the suspension. At any pH, the percentage sorption of (90)Sr was higher than that of ... [Abstract] [Full-text]

Articles on Radioisotopes published 13 April 2009:

Effect of pH, ionic strength and temperature on the sorption of radionickel on Na-montmorillonite.   Appl Radiat Isot, 67(6): 1007-12.

Sorption of Ni(II) on Na-montmorillonite was studied under ambient conditions using the batch technique. The effects of pH, solid content, ionic strength and temperature on Ni(II) sorption was also investigated. The kinetic sorption process was well described by a pseudo-second-order rate model. The sorption thermodynamics of Ni(II) on montmorillonite were carried out at 20+/-2 degrees C, 37+/-2 degrees C, and 67+/-2 degrees C, respectively. The thermodynamic parameters, such as standard free ... [Abstract] [Full-text]

Excitation functions of 3He- and alpha-particle induced nuclear reactions on natSb for production of medically relevant 123I and 124I radioisotopes.   Appl Radiat Isot, 67(6): 1001-6.

Excitation functions were measured using the stacked foil irradiation technique from threshold energies to 28 MeV for (3)He- and to 21 MeV for alpha-particle induced nuclear reactions on natural antimony leading to the formation of (121,123,124)I radioisotopes. The measured excitation functions were compared with the contradicting results of the earlier investigations found in the literature and with the curves predicted by the ALICE-IPPE and EMPIRE-II codes. Integral yields were also ... [Abstract] [Full-text]

Articles on Radioisotopes published 10 April 2009:

Study of the influence of radionuclide biokinetics on the efficiency of in vivo counting using Monte Carlo simulation.   Health Phys, 96(5): 558-67.

To improve calibration methods of in vivo counting, our laboratory has developed a computer tool to model internal contamination and assess in vivo activity and corresponding organ absorbed doses. The aim of the recent work was to define a more realistic source based on biokinetic models. The influence of the biokinetic parameters on the in vivo counting was studied through the simulation of an acute inhalation intake of (241)Am. The tissue distribution of activity predicted by the biokinetic ... [Abstract] [Full-text]

Articles on Radioisotopes published 9 April 2009:

Paradoxical exacerbation of preexisting Graves' disease induced by insufficient radioiodine treatment: a report of five patients.   Nucl Med Commun, 30(4): 275-80.

OBJECTIVE: This study reports on early and paradoxical aggravation of hyperthyroidism, which needs long-term treatment, in patients with Graves' disease after radioactive iodine (RAI) treatment. METHODS: Five patients (0.4%) out of 1333 consecutive patients with Graves' disease who underwent RAI treatment by using an empirical fixed dose of I between January 2000 and March 2006 revisited the emergency center because of early and markedly aggravated thyrotoxic manifestations, which seemed to ... [Abstract] [Full-text]

Thyroid remnant ablation in patients with papillary cancer: a comparison of low, moderate, and high activities of radioiodine.   Nucl Med Commun, 30(4): 263-9.

OBJECTIVE: The consensus about optimal activities of I-131 for thyroid remnant ablation has not yet been achieved. The aim of this study was to compare ablation rates obtained with different I-131 activities. PATIENTS AND METHODS: The study included 466 patients divided into four groups according to I-131 activities given after total thyroidectomy for papillary thyroid cancer: group A [168 patients who received 888 MBq (24 mCi)], group B [125 patients who received 1480 MBq (40 mCi)], group C ... [Abstract] [Full-text]

Articles on Radioisotopes published 6 April 2009:

Measurement of natural radionuclides in phosphgypsum using an anti-cosmic gamma-ray spectrometer.   Appl Radiat Isot, 67(5): 957-60.

Gamma-ray spectrometry measurements have been carried out to determine the activity of natural radionuclides in a phosphogypsum sample included in a specific tight container. The gamma spectrometer includes an N-type coaxial high-purity germanium (HPGe) detector equipped with an anti-cosmic system. This measurement required the determination of linear attenuation coefficients of phosphogypsum to calculate self-absorption correction between efficiency calibration conditions and measurement ones. ... [Abstract] [Full-text]

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